Fig. 9.

DPP3 activity correlates with estrogen status and lack of DPP3 augments bone loss after estrogen withdrawal. (A) Representative images of sections of decalcified paraffin-embedded femurs of SHAM and OVX WT mice stained with H&E (upper panels) and with anti-DPP3 antibody. Scale bar = 500 μm. (B) Evaluation of DPP3 enzymatic activity in SHAM and OVX WT mice in BM protein extracts; n ≥ 5 per group. (C) μCT analysis of the spine: 3D reconstruction and static histomorphometric analysis in SHAM and OVX WT and Dpp3 KO mice. For each evaluation, n ≥ 5 per genotype per group. Only statistically significant comparisons are depicted; mean values ± SD for all the parameters measured, as well as detailed statistical analysis are reported in Supporting Table 2. *p < .05, **p < .01.