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Immunostaining in the ovary of female Mythimna separata adults with polyclonal antiserum against Ms A-type mAChR protein. Streptavidin-peroxidase (SP) staining technique, cryosections. (A) Arrows showing the moderate positive signal at the membrane of the oocyte in the early stage of oogenesis. (B) The negative control, showing no positive signals. (C,D) Arrows the strong positive signal at the membrane of the oocytes and the old development oocytes. Scale bar = 200 μm.
