Fig. 5.

Comparison of sgDesigner with public sgRNA design tools. sgDesigner and three other algorithms were included in this analysis. Validation analysis was performed using six independent datasets, and the combined results are summarized here. Detailed results on each testing dataset are presented in Supplementary Figures S2 and S4. (a) ROC curve analysis. (b) Spearman correlations between experimentally determined efficiency and prediction score. (c) Summary of ROC–AUC values for sgDesigner on six independent datasets. (d) Summary of Spearman correlation coefficient values for sgDesigner on six independent datasets