Fig. 4.

Cytopathic effect of the cell-free supernatant of the C. novyi isolate in Vero 76 cells. Morphological alterations of Vero 76 cells exposed to cell-free supernatant of the C. novyi isolate for 5 days. a Vero 76 cells without treatment; the cells show a fibroblast-like morphology. b Vero 76 cells inoculated with filtered (0.2 μm) C. novyi supernatant (1:32). A cytopathic effect (rounding and disintegration of cells) was observed in cells treated with C. novyi supernatant