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. 2020 May 7;21:347. doi: 10.1186/s12864-020-6757-z

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© The Author(s). 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — The validation of the lncRNA and circRNA RNA-seq results. (a and b) the validation of the lncRNAs expression in LD and AA tissues. (c) Circ_040844 and Circ_053707 were amplified with RNase R-digested RNA or undigested RNA as templates. M. DL2000 DNA marker; “R+” indicates RNA treated with RNase R; “R-” indicates untreated RNA. (d and e) The Sanger sequencing of Circ_040844 and Circ_053707 RT-PCR products. The black arrows indicate head-to-tail back-splicing sites of circRNAs. (f and g). the validation of the circRNAs expression in LD and AA tissues. 0.5-LD: 0.5-year-old longissimus dorsi muscle tissues. 2.5-LD: 2.5-year-old longissimus dorsi muscle tissues. 0.5-AA: 0.5-year-old adjacent adipose tissue. 2.5-AA: 2.5-year-old adjacent adipose tissue