| Lifestyle |
Haploid, gametophyte-dominant |
| Annual |
| Grows axenically in simple mineral medium |
| Self-fertile (predominantly selfing) |
| Stress-tolerant |
| Genetics and genomics |
Can be crossed for genetic studies |
| Gene targeting by homologous recombination |
| Genome editing by CRISPR/Cas9 |
| Transfection of protoplasts and Agrobacterium-mediated transformation |
| Complete genomes, relatively small nuclear genome (500 Mbp, 27 chromosomes) |
| Transcriptome (expression profiling) data |
| Many mutants published |
| Genomes and primary annotation |
Nuclear genome assembly V3 for browsing at CoGe (including gene models v3.3, single nucleotide polymorphisms, RNA-seq expression evidence, TE and noncoding RNA annotation, microarray probes, DNA methylation, and histone modification) |
| https://genomevolution.org/coge/GenomeInfo.pl?gid=33928 |
| Nuclear, plastid, and mitochondrial genomes at CoGe |
| https://genomevolution.org/coge/OrganismView.pl?gid=33928 |
| Nuclear genome and gene models at Phytozome (including gene family assignment and RNA-seq expression evidence) |
| https://phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Ppatens |
|
P. patens gene model lookup database (for converting between different annotation versions) |
| https://peatmoss.online.uni-marburg.de/ppatens_db/pp_search_input.php |
| Online tools |
P. patens transcription factors and transcriptional regulators in TAPscan |
| https://plantcode.online.uni-marburg.de/tapscan/TAPs_for_species.php?species=Physcomitrella%20patens&id=98 |
|
P. patens expression atlas PEATmoss (including microarray and RNA-seq dataset) |
| https://peatmoss.online.uni-marburg.de/expression_viewer/input |
| Older expression atlas tools include Genevestigator (https://genevestigator.com/gv/) and an eFP browser (http://www.bar.utoronto.ca/efp_physcomitrella/cgi-bin/efpWeb.cgi) |
|
P. patens in the PLAZA comparative genomics environment |
| https://bioinformatics.psb.ugent.be/plaza/versions/plaza_v4_dicots/organism/view/Physcomitrella+patens |
| https://bioinformatics.psb.ugent.be/plaza/versions/plaza_v4_monocots/organism/view/Physcomitrella+patens |
|
P. patens in ENSEMBL plants |
| http://plants.ensembl.org/Physcomitrella_patens/Info/Index |
| Protocols and stocks |
Cold Spring Harbor protocols on cultivation of P. patens
|
| http://cshprotocols.cshlp.org/content/2009/2/pdb.prot5136.abstract |
| Protonema cultivation at Bio-protocol |
| https://bio-protocol.org/e1556 |
| PHYSCObase protocols |
| http://moss.nibb.ac.jp/protocol.html |
| International Moss Stock Center |
| http://www.moss-stock-center.org/ |
| Protocols on the Bezanilla Laboratory website |
| https://sites.dartmouth.edu/bezanillalab/moss-methods/ |
