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Disulfide Bond Formation Enhances the Activity of Reconstituted Plsp1 at Low pH.
(A) Proteoliposomes (PLs) containing T7-Plsp1 (wild type, or WT, or C286A) were resuspended in MES liposome buffer at pH 5.6 and were assayed for processing activity.
(B) Quantification of mature PsbP bands in (A) relative to wild type -TX-100. Shown are the means ± sd from three independent experiments. Blue = untreated PLs; Green = PLs + 2% TX-100. An asterisk denotes no detectable activity.
