Figure 3.

Cellular Trafficking Pathways Responsible for Recruiting PI(4,5)P₂ into the EHM.

Effects of pharmacological inhibitors on the targeting of PI(4,5)P₂ into the EHM are shown.

(A) Representative images showing the targeting of mCIT-1xPH^PLCδ1 to the EHM at 24 h post Ec inoculation after the indicated treatments. The leaves were infiltrated with mock (water), 5 µM latrunculin A (Lat-A), 1 mM oryzalin, 300 µM BFA, 1 mM MβCD, or 30 µM wortmannin 1 h inoculation with Ec. The haustorial neck regions are indicated by arrowheads. ha, haustorium. Bars = 10 μm.

(B) Quantification of relative fluorescence intensity for mCIT-1xPH^PLCδ1 at the EHM. Data are normalized over the intensity at the EHM from the mock treatment. Data are means ± sd (n = 30). Different letters indicate statistically significant differences determined by one-way ANOVA with Tukey’s HSD (P < 0.01).