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. 2020 Mar 10;32(5):1665–1688. doi: 10.1105/tpc.19.00970

Figure 5.

Figure 5.

Loss of PIP5K1 and PIP5K2 Functions Prevented Growth and Development of the Compatible Powdery Mildew Fungus.

(A) Macroscopic infection phenotypes of double (pip5k1 pip5k2), triple (pip5k1 pip5k2 pip5k5 and pip5k1 pip5k2 pip5k8), and quadruple (pip5k1 pip5k2 pip5k5 pip5k8) mutant plants at 10 DAI with Ec.

(B) Impaired growth and development of Ec on the indicated genotypes at 7 DAI with Ec. Leaf tissues were stained with aniline blue and viewed by light microscopy. Bars = 100 μm.

(C) Time course showing the development of Ec on mature leaves of the pip5k1 pip5k2 mutant. Leaf tissues of wild-type and pip5k1 pip5k2 plants at 2, 5, and 7 DAI were stained with aniline blue and viewed by light microscopy. Bars = 100 μm.

(D) Reduced formation of haustoria in mutant pip5k1 pip5k2. Fungal structures on the leaf surfaces (left) and haustoria in epidermal cells (right) at 7 DAI were stained with Alexa Fluor 488-conjugated wheat germ agglutinin (WGA), while callose deposition (middle) was detected by aniline blue. Images were taken with a confocal microscope with maximum projection of Z-stacks. Bars = 50 μm.

(E) to (I) Quantitative analysis of Ec growth on leaves of wild-type and pip5k1 pip5k2 plants. **, P < 0.01 and ***, P < 0.001, Student’s t test.

(E) Penetration rate of Ec. More than 100 sites for each leaf were scored at 2 DAI. Data are means ± sd (n = 4).

(F) and (G) Branch numbers (F) and total lengths (G) of secondary hyphae per colony at 2 DAI. Data are means ± sd (n = 75 [wild type] or 31 [pip5k1 pip5k2]).

(H) Haustorial numbers per colony at 2 DAI. Data are means ± sd (n = 31 [wild type] or 31 [pip5k1 pip5k2]).

(I) Number of conidiophores per colony at 7 DAI. Conidiophores were counted from at least 30 colonies in five leaves for each genotype, which was repeated three times with similar results. Data are means ± sd (n = 30).