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. 2020 Feb 28;32(5):1519–1535. doi: 10.1105/tpc.19.00455

Figure 3.

Figure 3.

SHR Movement into the Phloem Pole Is Required for Asymmetric Cell Divisions for Sieve Element Development.

(A) to (L) Confocal cross sections of the root meristematic zone of ProCRE1:SHRΔNLELDV-nlsGFP shr-2 (A) and ProS32:SHRΔNLELDV-nlsGFP shr-2 (D) show the GFP signal indicating the expression pattern of the nonmobile SHR protein. Toluidine blue–stained transverse sections and immunolocalization of the SE-ENOD of ProCRE1:SHRΔNLELDV-nlsGFP shr-2 ([B] and [C]) and ProS32:SHRΔNLELDV-nlsGFP shr-2 ([E] and [F]) are shown. Confocal cross sections of the root meristematic zone and immunolocalization of the SE-ENOD of ProEPM>>iCalsM3 ProSHR:SHR-GFP shr-2 ([G] to [L]) are also shown.

(G) to (I) Without an estradiol treatment, SHR movement and SE development are normal.

(J) to (L) Images taken 2 d after a treatment with 10 µM of estradiol. The phloem pole, indicated by white arrowheads, does not show SHR-GFP. Scale bar = 10 µm; asterisks, pericycle position; arrows, xylem axis; arrowheads, SEs.