Table 1. Statistics of the Genome-Editing Experiments.
Pool | Batch | sgRNAs | Vector No. (Vn)a | V′n in Plasmidb | T0 No. | Assigned T0 (Pn)c | V′n in T0 Plantsd | Genotyped Linese | Edited Lines |
---|---|---|---|---|---|---|---|---|---|
DSP | DSP1 | 90 | 49 | 48 | 157 | 125 | 38 | 95 | 79 |
DSP2 | 78 | 40 | 37 | 342 | 296 | 34 | 263 | 224 | |
DSP3 | 191 | 100 | 98 | 387 | 379 | 75 | 173 | 146 | |
DSP | 191 | 104 | 103 | 886 | 800 | 93 | 531 | 449 | |
SSP | SSP1 | 959 | 959 | 936 | 940 | 860 | 340 | – | – |
SSP2 | 1186 | 1186 | 320 | 1374 | 1016 | 257 | – | – | |
SSP3 | 1186 | 1186 | 1173 | 1156 | 1019 | 466 | – | – | |
SSP | 1186 | 1186 | 1178 | 3470 | 2895 | 685 | 1,290 | 693 | |
Total | 1368 | 1290 | 1281 | 4356 | 3695 | 778 | – | – |
Dashes indicate no data. Bold text represents the sum of each pool and the total of them.
Total vector number (Vn) pooled in this study.
Observed vector number (V′n) in plasmid pools.
T0 individuals successfully assigned to linked targets.
Vector number covered by those successfully assigned T0 individuals.
The number genotyped for DSP is indicated by the total T0 lines. The number of T1 lines with phenotypic change were selected for SSP genotyping (thus it is inappropriate and not used for estimation of general mutant ratio).