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. 2020 Feb 25;32(5):1397–1413. doi: 10.1105/tpc.19.00934

Table 1. Statistics of the Genome-Editing Experiments.

Pool Batch sgRNAs Vector No. (Vn)a V′n in Plasmidb T0 No. Assigned T0 (Pn)c V′n in T0 Plantsd Genotyped Linese Edited Lines
DSP DSP1 90 49 48 157 125 38 95 79
DSP2 78 40 37 342 296 34 263 224
DSP3 191 100 98 387 379 75 173 146
DSP 191 104 103 886 800 93 531 449
SSP SSP1 959 959 936 940 860 340
SSP2 1186 1186 320 1374 1016 257
SSP3 1186 1186 1173 1156 1019 466
SSP 1186 1186 1178 3470 2895 685 1,290 693
Total 1368 1290 1281 4356 3695 778

Dashes indicate no data. Bold text represents the sum of each pool and the total of them.

a

Total vector number (Vn) pooled in this study.

b

Observed vector number (V′n) in plasmid pools.

c

T0 individuals successfully assigned to linked targets.

d

Vector number covered by those successfully assigned T0 individuals.

e

The number genotyped for DSP is indicated by the total T0 lines. The number of T1 lines with phenotypic change were selected for SSP genotyping (thus it is inappropriate and not used for estimation of general mutant ratio).