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| parameter | condition |
|---|---|
| column | LiChrospher C18 (250 mm × 4.6 mm, 5 μm) |
| mobile phase | water:acetonitrile = 24:76 (% v/v) |
| elution | isocratic |
| flow rate | 0.75 mL/min |
| column temperature | ambient |
| wavelength for UV detection | 310 nm |
| run time | 12 min |
| retention time | ∼8.2 min |
| stock solution | acetonitrile |
| dilution | acetonitrile |
| software | LabSolutions |
