Fig. 3.

circ_0020710 acts as a sponge of miR-370-3p. a Schematic illustration exhibiting the overlap of circ_0020710 target miRNAs, predicted by miRanda, circbank, TargetScan, and RNAhybrid databases. b CircRIP was performed in A2058-circ_0020710 cells using circ_0020710 and negative control probes. c RIP experiments were carried out using an AGO2 antibody. d circ_0020710 and miR-370-3p were detected by FISH assay. e circ_0020710 level in the streptavidin-captured fractions from the melanoma cell lysates after transfection with biotinylated miR-370-3p or negative control. f Putative binding site of miR-370-3p with respect to circ_0020710 was predicated via StarBase v3.0. g The luciferase activity of pLG3-circ_0020710 in HEK-293 T cells after co-transfection with miR-370-3p. Unpaired student’s t-test and Mann-Whitney U test were used for the statistical analyses. **p < 0.01; ***p < 0.001; ns, no significant