Table 1.
Primer sequences designed for SYBR® Green real-time PCR.
| Gene name | Accession number | Sequence |
|---|---|---|
| T-bet-F | NM_013351.1 | 5′-ATTGCCGTGACTGCCTACCAGA-3′ |
| T-bet-R | 5′-GGAATTGACAGTTGGGTCCAGG-3′ | |
| GATA3-F | NM_001002295.1 | 5′-ACCACAACCACACTCTGGAGGA-3′ |
| GATA3-R | 5′-TCGGTTTCTGGTCTGGATGCCT-3′ | |
| ROR-γt-F | NM_005060.4 | 5′-CCCTGACAGAGATAGAGCACC-3′ |
| ROR-γt-R | 5′-TTCCCACATCTCCCACATGG-3′ | |
| FOXP3-F | NM_014009.3 | 5′-GGCACAATGTCTCCTCCAGAGA-3′ 5′-CAGATGAAGCCTTGGTCAGTGC-3′ |
| FOXP3-R | ||
| GAPDH-F | NM_001289746.1 | 5′-CACTAGGCGCTCACTGTTCTC-3′ |
| GAPDH-R | 5′-CCAATACGACCAAATCCGTTGAC-3′ |
Note: F: forward primer; R: reverse primer; GATA3: GATA binding protein 3; ROR-γt: retinoic acid-related orphan receptor gamma t; FOXP3: forkhead box P3; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.