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Effect of H2O2 on the mRNA expression of proinflammatory cytokines in IPEC-1 cells.
| Items1 | Control | 0.5 mM H2O2 | P value |
|---|---|---|---|
| IL-6 | 1.00 ± 0.05a | 4.89 ± 0.51b | <0.001 |
| IL-8 | 1.00 ± 0.05a | 4.19 ± 0.14b | <0.001 |
| TNF-α | 1.00 ± 0.03a | 6.51 ± 0.10b | <0.001 |
abMeans within a row differ significantly (P ≤ 0.05). 1The 2−ΔΔCt method was used to analyze the relative gene expression (fold changes), which was calculated relative to the values in samples from the control group.
