Table 4.

Effect of Bay11-7082 on mRNA expression of proinflammatory cytokines in IPEC-1 cells stimulated with H₂O₂.

Items1	-H2O2		+H2O2		P value
	Control	Bay11-7082	Control	Bay11-7082	Bay11-7082	H2O2	Interactions
IL-6	1.00 ± 0.10	0.50 ± 0.07	1.27 ± 0.02	0.78 ± 0.03	<0.001	0.002	0.898
IL-8	1.00 ± 0.03b	0.63 ± 0.02a	2.48 ± 0.15c	0.52 ± 0.06a	<0.001	<0.001	<0.001
TNF-α	1.00 ± 0.05a	0.80 ± 0.08a	15.75 ± 0.7c	3.05 ± 0.18b	<0.001	<0.001	<0.001

^a-cMeans within a row with different letters differ significantly (P ≤ 0.05). ¹The 2^−ΔΔCt method was used to analyze the relative mRNA expression (fold changes), calculated relative to the values in the control group.