Table 6.

Effect of PD98059 on the mRNA expression of proinflammatory cytokines in IPEC-1 cells stimulated with H₂O₂.

Items1	-H2O2		+H2O2		P value
	Control	PD98059	Control	PD98059	SB202190	H2O2	Interactions
IL-6	1.00 ± 0.11a	0.82 ± 0.1a	1.69 ± 0.11b	1.24 ± 0.14a	0.001	<0.001	<0.042
IL-8	1.00 ± 0.24a	0.88 ± 0.03a	27.90 ± 1.72c	22.64 ± 2.85b	0.024	<0.001	0.029
TNF-α	1.00 ± 0.10a	0.85 ± 0.23a	41.63 ± 5.69c	10.86 ± 1.74b	<0.001	<0.001	<0.001

^a-cMeans within a row with different letters differ significantly (P ≤ 0.05). ¹The 2^−ΔΔCt method was used to analyze the relative mRNA expression (fold changes), calculated relative to the values in the control group.