Figure 7.

Phagocytes contribute to autophagic clearance against ZIKV infection in vivo. (a) Neonatal mice were treated with rapamycin (3 mg/kg) and 3-MA (3 mg/kg) and then subcutaneously injected with ZIKV. Then, the mice were sacrificed and brain tissues were sectioned for immunostaining with ZIKV E-protein and F4/80 antibody. (b) ZIKV and F4/80 positive cells were counted in each vision. (c) Neonatal mice were infected with ZIKV or ZIKV-infected RAW264.7 cells, respectively. Three days after infection, the mice were sacrificed and brain tissues were sectioned for immunostaining with ZIKV E-protein and F4/80 antibody. Scale bar = 20 μm. (d) ZIKV- and F4/80-positive cells were counted. (e) The number of virus in the brain was counted using PFU assay. Means ± SD. One-way ANOVA; Tukey's post hoc test, ^∗p < 0.05. Data were representative from three mice of each experiment.