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. 2020 Jan 22;2020:8232734. doi: 10.1155/2020/8232734

Figure 5.

Figure 5

miR-129-5p served as a target of MEG3. (a) Binding sites between miR-129-5p and MEG3. (b) The luciferase reporter constructs containing the wild-type (WT-MEG3) or mutant MEG3 (MUT-MEG3) sequence. (c) WT-MEG3 or MUT-MEG3 was cotransfected into HEK-293T cells with miR-129-5p mimics or their corresponding negative controls. (d) The correlation between MEG3 and miR-129-5p was tested by RIP assay in Caco2 cells. Cellular lysates were immune-precipitated using Ago2 antibody or IgG. SNRNP70 level was employed as a positive control. Three independent experiments were carried out. Error bars stand for the mean ± SD of at least triplicate experiments. ∗P < 0.05.