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. 2007 Aug 1;196(3):405–415. doi: 10.1086/519166

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© 2007 by the Infectious Diseases Society of America

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 4. — The subcellular localization of open reading frame (ORF)-8a. A, HEK 293T cells transfected with plasmid pORF8a-EGFP. B, Severe acute respiratory syndrome coronavirus (SARS-CoV)-infected VeroE6 cells immunostained with preimmunized rabbit serum. C, SARS-CoV-infected VeroE6 cells immunostained with rabbit anti-ORF8a antiserum R26 (1:100 dilution). D-I, VeroE6 cells cotransfected with a mitochondrial expression plasmid (pEF-CKMt2-green fluorescent protein [GFP]) and pHA-ORF8a and immunostained with rabbit anti-ORF8a antiserum R26 (1:500 dilution). Rhodamine-conjugated goat anti-rabbit antibodies were used as a secondary antibody in the immunofluorescent antibody assay test. Green represents GFP (D and G); red represents ORF8a protein (E and H). Yellowish areas in merged panels (F and I) indicate ORF8a localized in mitochondria. D-F, Low magnification; G-I, high magnification.