Fig. 4.

Effect of SH3BGRL on breast cancer cell proliferation, cell cycle and apoptosis. a Dynamic analysis of SH3BGRL in downstream AKT and MAPK signaling pathways. The indicated cells were starved for 24 h, and treated with 20 ng/ml EGF for different time points (0, 5, 15 and 30 min), and the indicated proteins were immunoblotted. b Cell proliferation of MCF-7 cells by SH3BGRL ectopic expression and MDA-MB-453 cells by SH3BGRL knockdown were assayed by CCK8 methods. c Cell cycle progression of MCF-7 and MDA-MB-453 cells are shown. The right side is a representative flow cytometry histogram with the cell population percentage in each cell cycle phase presented as mean ± SEM. d Tumor formation induced by MCF-7 cells (Vector) and SH3BGRL overexpression cells (SH3BGRL), respectively. Cells were injected subcutaneously into flank sides of nude mice armpit. Tumors induced were harvested and weighed. The xenograft tumor weights were analyzed and shown in the lower panel, respectively, n = 3. e Tumor formation induced by MDA-MB-453 cells (Vector) and SH3BGRL knockdown cells (SH3BGRL KD). Cells were injected subcutaneously and analyzed as above. P < 0.0001, n = 7