Figure 1.

CoCl₂ induces iNOS, IL-1β, IL-6, and TNF-α mRNA expression in microglia. BV2 cells were treated with 300 μM of CoCl₂ for the indicated times (3, 6, 12, and 24 h). iNOS, IL-1β, IL-6, and TNF-α mRNA expression was detected by qPCR. The relative amounts of transcripts were calculated using the 2^−ΔΔCT formula. β-Actin mRNA was used as the internal control. (a) iNOS mRNA expression at different times. (b) IL-1β mRNA expression at different times. (c) IL-6 mRNA expression at different times. (d) TNF-α mRNA expression at different times. Data from at least three independent experiments are expressed as the mean ± S.E.M. ANOVA with the SNK post hoc test was used to assess differences between groups. ^∗P < 0.05, ^∗∗P < 0.01 versus the control group.