FIGURE 3.

Trunk mechanical hyperalgesia. Mean change from baseline of magnitude of mechanical trunk stimulus required to elicit a pain response (escape, withdraw behavior) following left VEH (50 μl; phosphate-buffer solution/PBS) or NGF (50 μl, 0.8 μM) injections (▲) and daily spinal mobilization (10 min; ∼0.9N). Female rats developed bilateral mechanical hyperalgesia that was prevented by daily spinal mobilization. Statistical analyses were performed by ANOVA followed by Tukey’s test at each time point and data are presented as mean ± SD (*P = 0.006, ^∧P = 0.04, ^#P = 0.03, ^Φ P = 0.001).