Figure 7.

JPYF II inhibits CSE-induced bronchial epithelial cell death through the ER stress-Ca²⁺ pathway. (A) Detection of the inhibitory effects of JPYF II and TUDCA on CSE-induced ER stress by Western blot. (B) Detection of the inhibitory effects of JPYF II and TUDCA on CSE-induced Ca²⁺ generation by flow cytometry. (C) Detection of the effects of JPYF II and TUDCA on CSE-stimulated BEAS-2B and 16-HBE cell viability by the MTT assay. (D) Detection of the inhibitory effects of JPYF II and BAPTA-AM on CSE-induced Ca²⁺ generation by flow cytometry. (E) Detection of the inhibitory effects of JPYF II and BAPTA-AM on CSE-induced ER stress by Western blot. (F) Detection of the effects of JPYF II and BAPTA-AM on CSE-stimulated BEAS-2B and 16-HBE cell viability by the MTT assay. (G) Detection of the inhibitory effects of JPYF II and 2-APB on CSE-induced Ca²⁺ generation by flow cytometry. (H) Detection of the inhibitory effects of JPYF II and 2-APB on CSE-induced ER stress by Western blot. (I) Detection of the effects of JPYF II and 2-APB on CSE-stimulated BEAS-2B and 16-HBE cell viability by the MTT assay. Results are presented by three independent experiments (n = 3). Values are presented as means ± SD. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 compared with control group; ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 compared with CSE group.