FIGURE 3.

Flux analysis of BMDMs incubated with heavy labeled substrates. BMDMs in PBS (red) or with IgG-opsonized RBCs (green) were uniformly labeled with ¹³C₆-glucose (A). Experimental results of ¹³C₆-glucose detection in BMDMs (B). Enrichment of the + 5 isotopologues of PPP metabolites, such as pentose phosphate isobaric isomers, were observed (top). Total percent labeled amounts of these isomers are shown in the bottom panel. These carbons are derived from glucose flux from the Embden-Meyerhof glycolytic pathway through the PPP. Continuous lines reflect the median of the three groups; whereas, the dashed lines represent interquartile ranges.