Figure 2.
Effect of LptE depletion on cell envelope stability. Lytic effect of different SDS concentrations (0–5%), measured as decrease in cell suspension turbidity (OD600), on PAO1 and lptE conditional mutant cells grown in the presence and/or absence of 0.5% arabinose (ARA), corresponding to the first (lptE) or second refresh (lptE 2nd) described in the legend of Figure 1. As control, LptH-deficient cells (lptH), obtained through the culturing strategy described in Fig. S6, were included in the analysis. Values are the mean (±SD) of three independent experiments performed in duplicate.