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. 2020 Apr 30;7:52. doi: 10.3389/fcvm.2020.00052

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Copyright © 2020 Xiang, Grosso, Takeda, Pan, Bekkhus, Brulois, Dermadi, Nordling, Vanlandewijck, Jalkanen, Ulvmar and Butcher.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In situ localization of Ptx3-LECs and transition between Ptx3-LECs and Marco-LECs in human LNs. (A) Expression of CD36/Cd36 in LN LEC subsets of human and mouse. Dots indicate mean log-normalized transcript count. (B–D) Identification of CD36^high Ptx3-LECs in human head and neck LNs by immunostaining. (B,C) Immunofluorescence of PROX-1, MARCO and CD36 (B), or PROX-1, LYVE-1 and CLEC4M (C). Zoomed-in images (inset marked by blue dotted lines) in (B) and (C) demonstrate CD36^high LYVE-1⁺ paracortical sinuses (filled arrowhead). Scale bars = 500 μm (left panels) and 100 μm (right panel inset). (D) CD36^high LYVE-1⁺ Ptx3-LECs (filled arrowhead) can be seen associated with MARCO⁺ CLEC4M⁺ Marco-LECs (empty arrowhead) in human LNs. Scale bars = 100 μm. CD36^high Ptx3-LECs were detected in four out of seven human LNs. Images are representative of four biological replicates.