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. 2020 Apr 21;8(1):e000673. doi: 10.1136/jitc-2020-000673

Figure 2.

Figure 2

ALKS 4230 induced the activation of intermediate-affinity IL-2R on human cells. Cells were incubated with increasing concentrations of ALKS 4230 or rhIL-2 for 30 min and were analyzed by flow cytometry for the percent pSTAT5+. (A) Human HH cells (lacking IL-2Rα) and (B) human NK-92 cells (expressing IL-2Rα), were treated with ALKS 4230 or rhIL-2 for 30 min and analyzed by flow cytometry. For C to E, equal amounts of blood were pooled from two each of healthy male and female donors ages 19 to 48, then leukocytes were isolated and incubated for 30 min with increasing concentrations of ALKS 4230 or rhIL-2. Cells were analyzed by flow cytometry for the percent pSTAT5+ cells in (C) NK cells, (D) effector memory CD8+ T cells, and (E) Tregs. Data are representative of one experiment with each sample being stimulated in triplicate. IL-2, interleukin-2; IL-2R, IL-2 receptor; NK, natural killer; pSTAT5, phosphorylation of STAT5; rhIL-2, recombinant human IL-2; Tregs, regulatory T cells.