Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 16;8(1):e000323. doi: 10.1136/jitc-2019-000323

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Analysis of the CD8⁺ T lymphocyte phenotype. (A) Gating strategy for CD45⁺CD3⁺ lymphocytes and subsequently CD8⁺ T lymphocytes. (B) Generated t-SNE plots of CD8⁺ lymphocytes based on the expression of H-2Kb-SIINFEKL tetramer, programmed cell death 1 (PD-1), TIGIT showed a clear distinction between different subsets and cell abundancies. (C) Gating strategy for different populations of CD8⁺ T lymphocytes, based on the subsets visualized in t-SNE plots. Gating was used to determine subset abundance and statistics. (D) Density overlaid on t-SNE plot show the relative cell density in each hemisphere. Manual gating overlaid on t-SNE plots show the location of defined subsets. Bar graphs show the frequency of each CD8⁺ T lymphocyte subset as a percentage of total CD45⁺CD3⁺ lymphocytes. Data are represented as mean±SEM. Statistical significance was assessed by analysis of variance followed by Tukey multiple comparisons test (*p<0.05, **p<0.005, ***p<0.001).