Table 1. SSRseq development strategy and DNA characteristics of species used in this study.

Kingdom	Class	Species	SSRseq development strategy	Number of DNA extracted individuals	DNA extraction protocol	DNA quality	Reference
Animalia	Actinopterygii	Salmo salar	Previously developed loci	1,152	Salt-chloroform (Gauthey et al., 2015)	High	Bacles et al. (2018) and Lepais et al. (2017)
Plantae	Eudicots	Quercus faginea, Q.canariensis	Re-designed primers around already developed loci using reference genome sequence of closely related species	380	Invisorb DNA Plant HTS 96 kit	High	This study
Animalia	Actinopterygii	Alosa alosa, A.fallax	De novo loci development based on available repeat-enriched library sequencing	382	Invitrogen PureLink Genomic DNA Mini kit	Highly degraded	Taillebois et al. (2020)
Fungi	Agaricomycetes	Armillaria ostoyae	De novo loci development based on reference genome sequence	384	CTAB (Prospero, Lung-Escarmant & Dutech, 2008)	Heterogeneous	This study
Animalia	Insecta	Melipona variegatipes	De novo loci development based on newly generated low coverage whole genome shotgun sequencing	91	Qiagen DNeasy 96 Blood & Tissue Kit	High	This study