Exosomes from Nischarin cells reduce tumor volume. A, Prkdcscid mice injected with Nisch+/+ or Nisch+/− cells, and Nisch+/+ or Nisch+/− cells previously cocultured with Nisch+/− exosomes. B, Tumor volumes of mice injected with Nisch+/+ (n = 3) exosomes, Nisch+/+ (n = 3) cells, or Nisch+/+ cells previously cocultured with Nisch+/+ (n = 3) or Nisch+/− (n = 3) exosomes. C, Tumor volumes of cells injected with Nisch+/− (n = 3) exosomes, Nisch+/− (n = 4) cells, or Nisch+/− cells previously cocultured with Nisch+/+ (n = 4) or Nisch+/− exosomes (n = 4). Red asterisks indicate statistical significance to the exosome groups. Black asterisks indicate statistical significance between the cells only group. D, Isolated mammary tumors from mice injected with Nisch+/+ or Nisch+/− cells, and Nisch+/+ or Nisch+/− cells previously cocultured with Nisch+/− exosomes. E, H&E staining of tumors. F, Ki67 staining of tumors with quantitation of the number of Ki67-positive cells per area (inset with a zoom is shown). G, Tumor stiffness (in kPa) of Nisch+/+ and Nisch+/− tumors. *, P < 0.05; **, P < 0.01; ***, P < 0.001.