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. Author manuscript; available in PMC: 2020 May 7.
Published in final edited form as: Commun Chem. 2019 Jul 3;2:77. doi: 10.1038/s42004-019-0172-x

Fig. 2.

Fig. 2

Interaction with furosemide and mitoNEET. a To evaluate an indirect binding effect of the interaction of furosemide with mitoNEET, a ELISA biotin–streptavidin capture assay shows that the furosemide–biotin probe is able to capture mitoNEET and be detected with a primary antibody; b the addition of furosemide (0–100 μM) is able to reduce the amount of mitoNEET captured from a N2A cell lysate by the furosemide-probe attached to streptavidin breads. Treatment: mitoNEET (mN); vehicle only (VC). RAU relative absorbance units