Table 3. In silico and viscosity properties of design mutants.
| Name | Heavy Chain | Light Chain | Fv Charge | Sharma Score | Tomar Score | SCM | Viscosity (cP) @ 100 mg/ml | Viscosity (cP) @ 150 mg/ml | Conc. (mg/ml) @ Visc. of 20 cP |
|---|---|---|---|---|---|---|---|---|---|
| AB-001 | AB-001_HC | AB-001_LC | -2.01 | 78.17 | 0.759 | -2213 | 40.4 ± 1.1* | 439.7 ± 127.8* | 82.9 ± 2.5 |
| R1-002 | E6Q_K94R | AB-001_LC | -1.07 | 76.87 | 0.778 | -2008 | 29.3 ± 0.9 | 288.3 ± 91.0* | 90.1 ± 3.5 |
| R1-003 | Q13K_K94R | AB-001_LC | -1.01 | 74.96 | 0.769 | -1985 | 39.5 ± 2.5* | 522.9 ± 255.9* | 84.9 ± 4.7 |
| R1-004 | A23R_K94R | AB-001_LC | -1.01 | 66.55 | 0.788 | -1961 | 36.0 ± 1.1 | 310.4 ± 94.4* | 85.1 ± 3.2 |
| R1-005 | D52aN_K94R | AB-001_LC | -1.04 | 77.92 | 0.73 | -1838 | 26.0 ± 1.5 | 190.2 ± 17.4* | 95.9 ± 3.0 |
| R1-006 | Q100bR_K94R | AB-001_LC | -1.01 | 71.16 | 0.653 | -1941 | 40.8 ± 1.1 | 313.7 ± 21.6* | 81.7 ± 2.0 |
| R1-007 | E6Q_Q13K_K94R | AB-001_LC | -0.07 | 77.86 | 0.777 | -1988 | 35.9 ± 5.4 | 233.2 ± 10.3 | 89.6 ± 3.7 |
| R1-008 | AB-001_HC | E3V | -1.07 | 73.93 | 0.529 | -2085 | 30.7 ± 1.0 | 567.2 ± 181.1* | 90.8 ± 3.0 |
| R1-009 | AB-001_HC | T18R | -1.01 | 59.05 | 0.507 | -2180 | 29.8 ± 0.8 | 430.4 ± 162.3* | 90.8 ± 5.1 |
| R1-010 | AB-001_HC | N53K | -1.01 | 64.64 | 0.602 | -1898 | 15.9 ± 0.5 | 98.6 ± 11.4* | 106.9 ± 5.1 |
| R1-011 | AB-001_HC | D96N | -1.04 | 67.85 | 0.607 | -2035 | 56.8 ± 2.5 | 519.4 ± 164.6* | 77.0 ± 2.2 |
| R1-012 | E6Q_Q13K_K94R | E3V | 0.87 | 67.48 | 0.639 | -1861 | 39.2 ± 1.5 | 471.0 ± 145.0* | 82.5 ± 3.5 |
| R1-013 | E6Q_K94R | T18R | -0.07 | 55.84 | 0.614 | -1972 | 29.7 ± 1.6 | 414.3 ± 285.4* | 90.9 ± 7.3 |
| R1-014 | E6Q_Q13K_K94R | T18R | 0.93 | 54.13 | 0.599 | -1983 | 27.5 ± 2.4 | 414.7 ± 116.6* | 89.2 ± 4.2 |
| R1-015 | E6Q_Q13K_K94R | D96N | 0.9 | 61.92 | 0.602 | -1817 | 41.3 ± 1.8 | 452.1 ± 211.4* | 82.6 ± 2.4 |
| R1-016 | E6Q_Q13K_K94R | N53K | 0.93 | 58.99 | 0.645 | -1706 | 14.5 ± 0.3 | 73.1 ± 0.6 | 109.0 ± 4.8 |
| R1-017 | Q13K_K94R | T18R | -0.01 | 54.41 | 0.594 | -1949 | 42.6 ± 2.8* | 1534 ± 1077* | 85.7 ± 4.5 |
| R1-018 | Q100bR_K94R | T18R | -0.01 | 51.83 | 0.559 | -1914 | 33.2 ± 0.6 | 415.8 ± 70.6* | 87.6 ± 2.7 |
| R2-001 | R2-001_HC | R2-001_LC | 3.87 | 28.09 | 0.221 | -1503 | 6.0 ± 2.0 | 37.3 ± 3.3 | 132.1 ± 7.2 |
| R2-004 | R2-001_HC | Y49H | 4.63 | 22.71 | 0.099 | -1480 | 14.8 ± 1.3 | 54.4 ± 2.0 | 110.8 ± 5.7 |
| R2-005 | R2-001_HC | Y49R | 4.87 | 19.43 | 0.078 | -1456 | 9.7 ± 5.9 | 36.7 ± 10.8 | 107.2 ± 17.7 |
| R2-006 | R2-001_HC | S52K | 4.87 | 20.74 | 0.072 | -1470 | 4.0 ± 1.2 | 12.6 ± 2.6 | 163.4 ± 21.7 |
| R2-007 | R2-001_HC | S65K | 4.87 | 20.74 | 0.054 | -1433 | 6.4 ± 3.0 | 21.1 ± 6.5 | 134.6 ± 17.5 |
| R2-008 | R2-001_HC | S67K | 4.87 | 20.74 | 0.062 | -1460 | 6.5 ± 2.7 | 23.0 ± 6.8 | 148.6 ± 16.5 |
| R2-009 | R2-001_HC | G68K | 4.87 | 20.18 | 0.076 | -1469 | 3.6 ± 0.7 | 19.3 ± 3.7 | 141.6 ± 10.6 |
| R2-010 | Y97H | R2-001_LC | 4.63 | 24.61 | 0.246 | -1656 | 9.6 ± 0.7 | 35.1 ± 1.6 | 122.6 ± 11.7 |
| R2-011 | Q100bK | R2-001_LC | 4.87 | 23.79 | 0.209 | -1500 | 13.1 ± 2.2 | 25.8 ± 2.4 | 131.3 ± 11.8 |
| R2-012 | D52aK | R2-001_LC | 5.85 | 20.9 | 0.226 | -1273 | 12.3 ± 3.3 | 38.9 ± 6.0 | 133.1 ± 5.7 |
| R2-013 | D52aN | R2-001_LC | 4.85 | 24.77 | 0.244 | -1357 | 7.1 ± 1.4 | 26.4 ± 4.3 | 133.2 ± 21.6 |
| R2-014 | D53Q | R2-001_LC | 4.85 | 24.68 | 0.17 | -1378 | 12.5 ± 0.8 | 50.8 ± 2.1 | 121.4 ± 5.0 |
| R2-015 | D61N | R2-001_LC | 4.85 | 24.77 | 0.237 | -1446 | 15.8 ± 1.9 | 83.2 ± 3.2 | 107.3 ± 6.0 |
| R2-016 | D101N | R2-001_LC | 4.85 | 24.77 | 0.231 | -1416 | 14.1 ± 1.2 | 66.5 ± 3.0* | 111.2 ± 5.0 |
| R2-017 | D101Y | R2-001_LC | 4.85 | 26 | 0.239 | -1564 | 8.8 ± 0.8 | 83.5 ± 2.8 | 115.4 ± 5.4 |
| R2-018 | R2-001_HC | D26N | 4.85 | 22.34 | 0.06 | -1346 | 3.4 ± 0.9 | 19.9 ± 7.1 | 127.4 ± 24.2 |
| R2-019 | R2-001_HC | D50L | 4.85 | 24.22 | 0.079 | -1503 | 23.2 ± 6.2 | 59.5 ± 6.6 | 91.3 ± 15.0 |
| R2-020 | R2-001_HC | D51N | 4.85 | 22.34 | 0.05 | -1363 | 4.1 ± 0.1 | 10.0 ± 0.2 | 182.4 ± 2.0 |
| R2-021 | R2-001_HC | D96K | 5.85 | 16.96 | -0.104 | -1197 | 24.2 ± 4.8 | 118.9 ± 10.9 | 100.2 ± 3.5 |
| R2-022 | R2-001_HC | D96N | 4.85 | 22.34 | 0.072 | -1311 | 25.2 ± 1.6 | 134.7 ± 2.6 | 98.5 ± 2.5 |
This table includes the identity of mutations, charge and in silico viscosity predictions from the Sharma, Tomar and SCM methods, the viscosities at 100 mg/ml, 150 mg/ml and the concentration at which the viscosity is 20 cP for the parental AB-001 and two rounds of design mutants. All sites are annotated with Kabat positions. Viscosities with (*) are extrapolated from the data fitting as the highest concentration obtained for this antibody was below 100mg/ml or 150 mg/ml respectively.