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. 2020 May 7;15(5):e0232754. doi: 10.1371/journal.pone.0232754

Fig 1. Complete NGS workflow for analysis of cfDNA, tumor tissue DNA, and PBMC DNA from patients with metastatic colorectal cancer.

Fig 1

(A) Workflow for analysis of cfDNA and gDNA from tumor tissues and PBMC. (B) Analysis of biologic background in all somatic variants of plasma cfDNA. cfDNA variants concordant with matched tissue are shown in red. cfDNA variants validated with the ddPCR platform are indicated with an ‘X’. (C) Correlation between NGS and ddPCR results of cfDNA VAF (n = 15, R2 = 0.94, Pearson’s ρ = 0.97). cfDNA, cell-free DNA; ddPCR, droplet digital polymerase chain reaction; FFPE, formalin-fixed, paraffin-embedded; gDNA, genomic DNA; NGS, next-generation sequencing; PBMC, peripheral blood mononuclear cells; VAF, variant allele frequency.