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. 2020 Apr 9;5(7):e136041. doi: 10.1172/jci.insight.136041

Figure 5. Confocal micrographs of vertical sections of RNAscope in situ hybridization.

Figure 5

Vertical section triple labeled with Arr3 (green), ATF6 (red), and ATF6B (white) mRNA in normal human retina. Nuclei were labeled with DAPI (blue) to identify the nuclear layers of the retina. The mRNA probe for Arr3 was found in the cell bodies and inner segments of cones, and the mRNA probes for ATF6 (red) and ATF6B (white) were observed in all the retinal layers. In the merged image, ATF6 and ATF6B mRNA puncta were localized within the cell bodies that are labeled with Arr3 in the outer nuclear layer (ONL). Three sets of independent experiments were performed (n = 2 retinas). Expression of ATF6 and ATF6B puncta was localized in the ONL, outer plexiform layer (OPL), inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL). Scale bar: 10 μm.