Figure 3. Multilineage PBMC donor chimerism.

Donor-specific multilineage mixed chimerism was detected in animals treated with CoB, indicating viable donor hematopoiesis derived from the graft inherent donor BM compartment. The addition of TBI further increased mixed chimerism levels (n = 3–7/time point). (A) Donor-derived CD3⁺ T cells. (B) Donor-derived B220⁺ B cells. (C) Donor-derived CD11b⁺ myeloid cells. The percentage of expression of donor MHC class I for each lineage circulating in host peripheral blood was calculated. Data are representative of at least 3 mice per group from 2–3 independent experiments.