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. 2020 Apr 9;5(7):e131486. doi: 10.1172/jci.insight.131486

Figure 11. NFATC4 overexpression inhibits MYC, and MYC overexpression partially rescues the quiescent phenotype at early, but not late, time points.

Figure 11

(A) MYC mRNA expression following 24-hour doxycycline treatment. SKOV3 (n = 4), HEY1 (n = 3). (B) Validation of MYC overexpression construct in SKOV3 and HEY1 cell lines (n = 3). (C) The effect of MYC overexpression on cell number of IcNFATC4 cells transfected with pcDNA3 or MYC and treated with doxycycline for 72 hours (SKOV3 n = 5; HEY1 n = 3) or posttreated for 72 hours (SKOV3 n = 4; HEY1 n = 3) with doxycycline before transfection with pcDNA3 or MYC and treated with doxycycline for an additional 72 hours. T tests and 1-way ANOVAs were performed to determine significance. n.s., not significant; *P < 0.05; **P < 0.01; ****P < 0.0001.