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. 2020 Apr 9;5(7):e131486. doi: 10.1172/jci.insight.131486

Figure 5. NFATC4 overexpression significantly inhibits cell growth.

Figure 5

Cell counts of (A) A2780 (n = 4) cell line or (B) HGSC cell lines (COV362 n = 7, OVSAHO n = 4, and CaOV3 n = 4, at 6, 4, and 6 days, respectively) expressing cNFATC4 or control-YFP constructs. (C) Cell counts of HEY1 (n = 3) and SKOV3 (n = 4) cells expressing IcNFATC4 or ILuc control constructs treated with or without doxycycline. (D) Trypan blue viability staining of A2780 (n = 6) cells expressing cNFATC4 or YFP control or SKOV3 (n = 3) cells expressing IcNFATC4 or ILuc control with or without 100 ng/mL doxycycline. (E) Representative images of annexin V/PI staining of A2780 cells expressing cNFATC4 or YFP control (n = 3). T tests and 1-way ANOVAs were performed to determine significance. *P < 0.05; **P < 0.01; ***P < 0.001.