Figure 6. NFATC4 inhibits proliferation and arrests cells in G₀.

(A) Quantitation of BrdU incorporation of A2780 cells expressing control-YFP (n = 8) or cNFATC4 (n = 10). (B) IncuCyte growth curves of IcNFATC4 or ILuc cells treated with or without doxycycline (n = 4). (C) FACS plots demonstrating the isolation and quantification of IcNFATC4 and ILuc HEY1 cells expressing the fluorescence ubiquitination cell cycle indicator (FUCCI) cell cycle reporter vectors (n = 3). Bar graph summarizing the cell cycle phase of cells expressing either construct. (D) Cell cycle analysis of G₁/S phase enriched ILuc and IcNFATC4. T tests and 1-way ANOVAs were performed to determine significance. Scale bars: 100 μm. ****P < 0.001.