Figure 1. Effect of sPRR-His on body weight and metabolism in diet-induced obesity in male C57/BL6 mice.

Starting from 1 month of age, male C57/BL6 mice were placed on a high-fat diet for 9 months. During the last 2 weeks, mice were randomly divided to receive vehicle or sPRR-His. (A) The body weight changes over the 2-week treatment period. (B) The ratio of epididymal fat weight to body weight. (C) Plasma volume. (D) Food intake normalized by body weight. (E) Water intake normalized by body weight. (F) VO₂ normalized by body weight. (G) VCO₂ normalized by body weight. (H) Heat production normalized by body weight. (I) Respiratory exchange ratio (RER). For A–C, n =10. For D–I, n = 5. *P < 0.05 vs. DIO group by using ANOVA with the Bonferroni test for multiple comparisons. Data are shown as mean ± SEM.