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. 2020 Apr 9;5(7):e133785. doi: 10.1172/jci.insight.133785

Figure 3. miR-214 deletion augments cyst-associated inflammation.

Figure 3

(A) Volcano plot showing 972 differentially expressed genes between Pkd2-KO miR-214–/– and Pkd2-KO kidneys (n = 3/group). (B) Unbiased pathway analysis revealed that inflammation-associated pathways are among the top dysregulated pathways in Pkd2-KO miR-214–/– kidneys compared with Pkd2-KO kidneys. (C) Q-PCR analysis showed that the expression of M2-like macrophage markers Mrc1, Ccl2, Arg1, and Ym1 is increased in Pkd2-KO miR-214–/– kidneys (n = 8) compared with Pkd2-KO kidneys (n = 8). Western blot (D) and quantification (E) demonstrated increased MRC1 expression in Pkd2-KO miR-214–/– kidneys (n = 9) compared with Pkd2-KO kidneys (n = 9). (F) Immunofluorescence staining revealed enhanced accumulation of MRC1+ macrophages (red) surrounding cyst epithelial cells in Pkd2-KO miR-214–/– kidneys (n = 5). The sections are costained with DAPI (blue). *P < 0.05; Student’s unpaired t test (C and E); error bars indicate SEM.