Figure 7. In vivo selectivity to HER2-overexpressing tumors in mice supports therapeutic index for anti-HER2/CD3 TDB.

(A) In the 2-tumor model, each NSG mouse was implanted with HER2-amplified KPL4 tumors and HT55 tumors, which express low levels of HER2. Mice were further supplemented with human PBMCs by i.p. injection. HER2 expression was analyzed by Western blot. Lanes were run on the same gel but were noncontiguous (n = 1). (B) Mice with 2 established tumors were treated with single 0.05 mg/kg dose of HER2–TDB 1. n = 5 for each group. Data represented as mean ± SEM.