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. 2020 May 7;15(5):e0232360. doi: 10.1371/journal.pone.0232360

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© 2020 Strahl, Abele

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Imaging of gill filaments of Arctica islandica 5 μm above the z-depth (A), and in the z-depth of the blood vessel (B); Nitric oxide (NO) formation in gills is expressed as the DAF-2T fluorescence intensity ratio between hypoxic and control pO₂ (C; n = 5–6). A1&B1: Overview of gill-filament structure (fluorescence image), showing nuclei in green (Syto-13 staining) and mitochondria in red (Mitrotracker Deep Red 633 staining); A2&B2: Representative NO formation in gill filaments indicated as green fluorescence (DAF-2T); A3&B3: Transmission image. The white box represents the region of interest (ROI) in the gill filaments, one layer above the z-depth analyzed for NO formation; bold white arrows indicate nuclei; pink arrows indicate blood vessel in the z-depth of the filament; epc = epithelial cells; elc = endothelial cells.