Fig 1. Groups of patients included in this study.

It was defined the cases or non-cases of American Tegumentary Leishmaniasis (ATL) from suspected patients by four diagnostic methods mentioned above. Three of those 129 subjects were inconclusive and could not be grouped. Additionally, for the non-ATL cases, there were included two groups of donors without ATL suspicion: Chagas seropositives and Japanese donors from non-endemic geographic area for ATL and Chagas disease.