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. 2020 May 7;15(5):e0232829. doi: 10.1371/journal.pone.0232829

Fig 2. Comparison between the main local species, promastigoste vs. amastigote extracts and membrane based vs. soluble extracts.

Fig 2

Comparison between Optical Density (OD) for each ATL case and resultant absorbance means (ODm) obtained from the following three consecutive assays, showing the differences among the avidity for anti-Leishmania antibodies. A) The ODs of 17 ATL cases and its ODm obtained by ELISA using promastigote Ags of five Leishmania zymodemes (0.15 μg/well), sera (1/2000,100 μl/well), and conjugate (1/500) conditions [*p by ANOVA/Dunn´s multiple comparison test vs. main local L. (V.) braz]; B) The ODs of 21 ATL cases obtained by using amastigote or promastigote Ags from Argentinean L. (V.) braziliensis main zymodeme (0.15 μg/well), sera (1/2000,100 μl/well), and conjugate (1/500) [*p by Wilcoxon test]; C) The ODs of 21 ATL cases obtained by using membrane or soluble extracts from amastigotes of Argentinean L. (V.) braziliensis main zymodeme under conditions previously described [*p by Wilcoxon test]. A p-value less than 0.05 was considered significant.