Fig 3. Diagnostic performance of the selected antigenic extract.

Enzyme Lynked Immunosorbent Assay reactivity using the membrane amastigote extract against ATL and non-ATL cases sera Aand B, and excluding Chagatest reactive samples, C and D. The relation ${\overline{OD}}_x$ / CO, where ${\overline{OD}}_x$ is the average of duplicate or triplicate ODs for each sample tested, and CO = Cut-off. The indetermination area (IA) is shown in dotted line, and defined as CO / CO±10%CO / CO = 0,9–1,1. B and D show the Receiver Operating Characteristic (ROC) Curves for ELISAs.