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(A) Self-association of Tm-22 was induced by TMV MP effector. (B) Tm-22 (D481V) auto-active mutant self-associated in the absence of TMV MP. (C) Functional P-loop motif was required for TMV MP-induced Tm-22 self-association. Indicated protein samples were subjected to anti-Myc immunoprecipitation (IP), followed by immunoblotting (IB) using the indicated antibodies. Protein molecular weight (kDa) marker is indicated on the right.
