Fig 4. Enhanced self-association promotes Tm-2² CC-mediated cell death.

(A) Cell death mediated by CC fused with YFP-Rop or monomeric YFP-Rop (mYFP-Rop). The leaves were stained by trypan blue to facilitate observation of cell death. (B) The confocal images of CC-mYFP-Rop. Bar = 50 μm. (C) The protein levels of different fusion proteins. Rubisco L (RbcL) was stained by Ponceau S as loading control. (D) Cell death mediated by CC fused with dimerization peptide (DP) or mutant DP. CC fused to DP exhibited stronger cell death than CC fused to mDP. (E) DP enhanced the self-association of CC fusion proteins. Myc-Rop or YFP-Rop tagged CC fusion proteins were transiently expressed in pair. Proteins extracted from leaves were subjected to IP with anti-GFP beads, followed by IB using anti-Myc or anti-GFP antibody.