Fig 6. Nucleotide binding state switches CC-NB-ARC activation.

(A) Self-association ability of CC, CC-NB-ARC or CC-NB-ARC (D481V). All domains were fused with Myc-Rop or YFP-Rop. CN stands for CC-NB-ARC. After co-expression, protein extracts were subjected to IP with anti-GFP beads, followed by IB with anti-GFP or anti-myc antibody. (B) and (C), K191R mutation repressed cell death signaling and impaired self-association of CC-NB-ARC (D481V)-Rop. Indicated protein samples were subjected to IP with anti-GFP beads, followed by IB with anti-Myc or anti-GFP antibody.