Table 2. Median frequencies of chromosome instability in cdc13F684S mutants at 30°C.
| Strain | Sectored (×10−5) | Round (×10−5) | Chr. Loss (×10−5) | |
|---|---|---|---|---|
| Wild Type (CDC13+) | 2.9 [3.4] (1.0) | 7.1 [4.5] (1.0) | 12 [38] (1.0) | |
| cdc13F684S | 88 [120] (30) | 20 [20] (2.8) | 73 [93] (6.1) | |
| Nucleases | exo1Δ | 11 [13] (3.8) | 48 [15] (6.8) | 44 [100] (3.7) |
| cdc13F684S exo1Δ | 5.7 [3.8] (2.0) | 380 [69] (54) | 20 [21] (1.7) | |
| sae2Δ | 7.3 [2.6] (2.5) | 10 [7.7] (1.4) | 32 [33] (2.7) | |
| cdc13F684S sae2Δ | 35 [16] (12) | 8.9 [5.5] (1.3) | 89 [55] (7.4) | |
| RPA mutants | rfa1-t33 | 260 [96] (90) | 20 [13] (2.8) | 1300 [450] (110) |
| cdc13F684S rfa1-t33 | 270 [55] (93) | 10 [4.6] (1.4) | 870 [390] (73) | |
| rfa1-t33 sae2Δ | 130 [17] (45) | 3.9 [3.8] (0.55) | 910 [260] (76) | |
| HR | rad52Δ | 16 [4.7] (5.5) | < 1 | 14 [11] (1.2) |
| cdc13F684S rad52Δ | 230 [320] (79) | < 1 | 1200 [1500] (100) | |
| NHEJ | lig4Δ | 2.8 [2.1] (1.0) | 8.6 [6.9] (1.2) | 12 [13] (1.0) |
| cdc13F684S lig4Δ | 74 [120] (26) | 16 [30] (2.3) | 12 [31] (1.0) | |
| DNA damage checkpoint | rad9Δ | 54 [35] (19) | 8.0 [4.6] (1.1) | 35 [130] (2.9) |
| sae2Δ rad9Δ | 87 [52] (30) | 28 [16] (3.9) | 290 [290] (24) | |
| cdc13F684S rad9Δ | 280 [330] (97) | 12 [8.2] (1.7) | 960 [1100] (80) |
Median values and IQR, in []s, are reported.
In bold, statistically significant (P < 0.01; Mann-Whitney U) fold change between single mutants and Wild Type (CDC13+) or between cdc13F684S mutX and cdc13F684S (except for rfa1-t33 sae2Δ and sae2Δ rad9Δ, which are in relation to rfa1-t33 and rad9Δ respectively).
Normalized median frequencies are reported in ()s (normalized to CDC13+).